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Primer sequences used for RT-PCR reaction
| Primer name | Sequence (5'-3') |
|---|---|
| miR-34aM-F: | GGTTTTGGGTAGGCGCGTTTC |
| m i R-34a M -R: | TCCTCATCCCCTTCACCG CCG |
| miR-34aU-F: | IIGGTTTTGGGTAGGTGTGTTTT |
| m i R-34a U -R: | AATCCTCATCCCCTTCACCACCA |
| AR-F: | TCCAGGATGCTCTACTTCGCCCC |
| AR-R: | CCGGGACTTGTGCATGCGGT |
| PSA-F: | GCAGTCTGCGGCGGTGTTCT |
| PSA-R: | TGGGCAGCTGTGAGGACCCA |
| Beta-actin-F: | CCCGCCGCCAGCTCACCATG |
| Beta-actin-R: | CGACGACGAGCGCGGCGATA |
M: methylation, U: unmethylation, F: forward, R: reverse.
