Table 1.
Sequence Statistics and RNA qualities
| Samples | Non- globin reduced | Globin-reduced | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 1 | 2 | 3 | 4 | 5 | 6 | |
| RIN | 8.1 | 7.7 | 7.1 | 6.9 | 7.3 | 8.8 | 7.6 | 7.2 | 6.7 | 6.9 | 7 | 8.2 |
| Tag numbers | 29.2.106 | 13.2.106 | 32.0.106 | 11.6.106 | 13.6.106 | 10.1.106 | 16.5.106 | 28.7.106 | 15.9.106 | 15.3.106 | 15.9.106 | 14.9.106 |
| Non- aligned | 1.27% | 5.63% | 1.43% | 13.80% | 5.64% | 1.92% | 3.64% | 2.95% | 3.37% | 3.29% | 3.45% | 3.38% |
| Aligned | 89.9% | 84.4% | 89.9% | 80.1% | 89.1% | 91% | 78.3% | 78.9% | 78.4% | 78.9% | 77.6% | 77.5% |
| Globin tags | 60.1% | 52.1% | 57.2% | 69.6% | 76% | 71% | 0.3% | 0.4% | 0.4% | 0.2% | 0.4% | 0.1% |
Quality of RNA depicted by RNA integrity numbers (RIN) for the 6 non-globin reduced and globin reduced samples. The number of tags, percentages of aligned and non-aligned sequences, and relative number of globin tags are shown as percentage of the total number of aligned tags.