Table 2. Comparative densitometric analysis of the band intensity of FoxP3 mRNA.

Average intensity
Set-I	Healthy PBMCs	Patients PBMCs
GAPDH	6.43	5.76
FoxP3	0.86	2.133
**Ratio (r)	0.135	0.369
Relative ratio	1	2.7

Ratio of relative intensity shows 2.7 times increase in band intensity of FoxP3 mRNA in patient PBMCs as compared to healthy PBMCs. FoxP3 band intensity is 2.28 times higher among patient's BMMNCs compared to their PBMCs. In cured cases, band intensity is 2.75 times higher than its pre-treatment level. Set I experiments were executed with higher amount of DNA compared to set II, due to extremely lower expression of FoxP3 in healthy subjects. (*Between Patient's PBMCs and BMMNCs at pretreatment level, # Between Patient's BMMNCs at post treatment level, ** r = average intensity of FoxP3 bands/GAPDH bands).