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. 2012 Feb 8;7(2):e31224. doi: 10.1371/journal.pone.0031224

Figure 4. Hif2p, Set3p, and Snt1p form a nuclear-localized complex.

Figure 4

(A) Cells expressing the indicated fusion proteins were grown to mid-log phase at 30°C in YES media, fixed, and then stained with DAPI and observed using the DAPI and GFP filter sets. Bar, 5 µm. (B) Cells expressing the indicated fusion proteins were grown to mid-log phase in YES, lysed under native conditions, and subjected to anti-Myc immunoprecipitations. Both total lysates and immunoprecipitates were resolved by SDS-PAGE and immunoblotted with antibodies specific for the HA epitope.