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. 2012 Feb 8;7(2):e31224. doi: 10.1371/journal.pone.0031224

Figure 5. Set3p, Snt1p and Hif2p levels increase two- to three-fold upon treatment with low doses of LatA.

Figure 5

(A) Strains expressing the indicated fusion proteins were grown to early log phase in YES at 30°C and treated with 0.5 µM LatA (left panel) or 1 µM LatA (right panel). Extracts were subjected to SDS-PAGE, transferred to PVDF membranes, and immunoblotted with anti-HA antibody. Tubulin was used as a loading control. (B and C) Strains expressing the indicated fusion proteins were grown to early log phase in YES at 30°C and treated with 0.5 µM LatA. Extracts were subjected to SDS-PAGE, transferred to PVDF membranes, and immunoblotted with anti-HA antibody. Tubulin or the RNA pol II carboxy-terminal domain (CTD) were used as loading controls. Signal intensities relative to the loading controls of three independent trials were quantified using ImageJ 1.41 Gel Analyzer software and are plotted below representative blots. Error bars indicate sd.