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. 2012 Feb 8;7(2):e31638. doi: 10.1371/journal.pone.0031638

Table 1. Toxicity of Hoechst and mercury lamp for mouse embryo development.

In vitro development at 96 h
Type of embryos Hoechst staining Mercury light No. used oocytes No. activated/fertilized oocytes 1, 2-cell 8-cell Blastocyst
Partheno. 119 87 5 (5.7) 18 (20.7) 64 (73.6)
+ 117 47 2 (4.3) 18 (38.3) 27 (57.4)
+ 129 117 49 (41.9) 46 (39.3) 18 (15.4)
+ + 141 62 51 (82.3) 7 (11.5) 0 (0.0)
ICSI 98 82 8 (9.8) 26 (31.7) 48 (58.5)
+ 93 26 7 (26.9) 6 (26.1) 10 (43.5)
+ 99 50 6 (12.0) 23 (46.0) 21 (42.0)
+ + 100 24 13 (54.2) 10 (43.5) 0 (0.0)
Clone 96 73 62 (85) 55 (75) 38 (52.1)
+ 108 76 53 (70) 36 (47) 1 (1.3)
+ 114 72 55 (76) 47(65) 24 (33.3)
+ + 105 79 48 (61) 30 (38) 4 (5.1)