Table 1. Toxicity of Hoechst and mercury lamp for mouse embryo development.
In vitro development at 96 h | |||||||
Type of embryos | Hoechst staining | Mercury light | No. used oocytes | No. activated/fertilized oocytes | 1, 2-cell | 8-cell | Blastocyst |
Partheno. | − | − | 119 | 87 | 5 (5.7) | 18 (20.7) | 64 (73.6) |
+ | − | 117 | 47 | 2 (4.3) | 18 (38.3) | 27 (57.4) | |
− | + | 129 | 117 | 49 (41.9) | 46 (39.3) | 18 (15.4) | |
+ | + | 141 | 62 | 51 (82.3) | 7 (11.5) | 0 (0.0) | |
ICSI | − | − | 98 | 82 | 8 (9.8) | 26 (31.7) | 48 (58.5) |
+ | − | 93 | 26 | 7 (26.9) | 6 (26.1) | 10 (43.5) | |
− | + | 99 | 50 | 6 (12.0) | 23 (46.0) | 21 (42.0) | |
+ | + | 100 | 24 | 13 (54.2) | 10 (43.5) | 0 (0.0) | |
Clone | − | − | 96 | 73 | 62 (85) | 55 (75) | 38 (52.1) |
+ | − | 108 | 76 | 53 (70) | 36 (47) | 1 (1.3) | |
− | + | 114 | 72 | 55 (76) | 47(65) | 24 (33.3) | |
+ | + | 105 | 79 | 48 (61) | 30 (38) | 4 (5.1) |