Table 2. Production of cloned mice using phycoerythrin and antibody injected oocyte.
Conc. of antibody (µg/ml) | No. used oocyte | No. survived after antibody injection | No. survived after enucleation | No. survived after nuclear injection | No. survived after activation | No. pronuclear formation | No. embryo developed to 2-cell | No. offspring (% against pronuclear egg) |
0 | 197 | — | 197 | 189 | 178 | 170 | 146 | 13 (7.6) |
75 | 148 | 141 | 140 | 137 | 128 | 117 | 108 | 8 (7.0) |
150 | 182 | 171 | 169 | 157 | 151 | 146 | 127 | 7 (4.8) |
300 | 163 | 159 | 157 | 135 | 119 | 115 | 105 | 7 (6.0) |
Total | 690 | 471 | 663 | 618 | 576 | 548 | 486 | 35 (6.4) |