Table 1. List of primers used for oligonucleotide-directed mutagenesis.
SNP | Amino acid change | Sequence | Tm (°C ) |
---|---|---|---|
rs1048661 |
R→L |
F-5’- gggcatggcccTggcccgcacctcc -3’ |
76 |
|
|
R-5’- ggaggtgcgggccAgggccatgccc -3’ |
76 |
rs3825942 |
G→D |
F-5’- acggcacggggActccgcctcctcg -3’ |
75 |
R-5’- cgaggaggcggagTccccgtgccgt -3’ | 75 |
In the sequence, the bases mismatched with the template are indicated in capital letters.