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. 2012 Jan 31;18:265–270.

Table 1. List of primers used for oligonucleotide-directed mutagenesis.

SNP Amino acid change Sequence Tm (°C )
rs1048661
R→L
F-5’- gggcatggcccTggcccgcacctcc -3’
76
 
 
R-5’- ggaggtgcgggccAgggccatgccc -3’
76
rs3825942
G→D
F-5’- acggcacggggActccgcctcctcg -3’
75
    R-5’- cgaggaggcggagTccccgtgccgt -3’ 75

In the sequence, the bases mismatched with the template are indicated in capital letters.