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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Dev Dyn. 2011 Aug 30;240(10):2344–2353. doi: 10.1002/dvdy.22724

Figure 4.

Figure 4

Early and late EC marker positive cells are found in E9.5 mouse PE. A–E Immunofluorescence analysis of sagittal sections from E9.5 22–24 somite (22–24S) mouse embryos. A–C Sections were stained with the PE marker WT1 (green), LB marker Prox1 (blue), EC markers A Flk1 (red) B VE-Cadherin (red) C PECAM-1 (red). D–E Flk1 and VE-Cadherin positive cells also co-express PECAM-1 in the E9.5 mouse PE. Sections were stained with WT1 (blue), PECAM-1 (green) and D FLK1 (red) E VE-Cadherin (red). White dashed line demarks the border of the heart. Yellow dashed line demarks the border of the LB. Scale bars = 50μm