Figure 1.

Regulation of glial and neuronal migration by LCN2. Microglia, astrocytes, or neuronal cells (1×10⁴ cells/upper well) were exposed to control (CTRL) or LCN2 (10µg/ml)-stimulated astrocyte-conditioned media (ACM) as indicated. Microglia, astrocytes, or neuronal cells placed in the Boyden chambers were incubated at 37℃ for 48 hr to evaluate cell migration. CTRL-ACM, untreated ACM; LCN2-ACM, LCN2-treated ACM (see Materials and Methods for the preparation of ACM). The quantification of cell migration was done by enumerating the migrated cells as described in the Materials and Methods section. The results are mean±SD (n=3). ^*p<0.05 compared with CTRL-ACM.