Figure 2.

NDRG2 induces GATA-1 expression. (A) U937-mock and U937-NDRG2 cells were treated with 0.1µg/ml PMA for 48 h. mRNA levels for GATA-1 and NDRG2 were measured by RT-PCR. β-actin was used as a loading control. (B) Equal amounts of whole protein lysates were subjected to electrophoresis on SDS-PAGE gels, and Western blot analysis was performed using specific antibodies. Actin was used as an internal loading control. (C) Expression of genes promoting the differentiation of erythrocyte and megakaryocyte. U937-mock and U937-NDRG2 cells were treated with 0.1 µg/ml PMA for 48 h prior to RT-PCR analysis.