Fig. 3.

Dendrogram of the DGAT2 and ACAT membrane-bound families. The protein sequences deduced from the various full-length cDNAs of species belonging to three different kingdoms were aligned and bootstrapped with the ClustalX program (www.clustal.org). The tree was then generated using the NJplot program (Perrière and Gouy 1996). The proteins sequences used were DGAT1 (NP03611.2), DGAT2 (NP115953.1), and ACAT1 (NP003092.4) sequences from humans (Homo sapiens); DGAT1 (NP034176.1), DGAT2 (NP080660.1), and ACACT (NP033256.2) from the mouse (Mus musculus); the DGAT1 (XP002514132.1) and DGAT2 (XP002528531.1) sequences from the castor bean (Ricinus communis); the DGAT1 (NP179535.1) and DGAT2 (NM115011.3) sequences from Arabidopsis (Arabidopsis thaliana); the DGAT1 (AAG23696.1) sequences from the plant P. frutescens; the DGA1 (NP014888.1), ARE1 (CAY78257.1), and ARE2 (NP014416.1) sequences from the baker’s yeast (S. cerevisiae); and the DGA1 (XP504700.1), DGA2 (XP502557.1), and ARE1 (XP505086.1) sequences from Y. lipolytica. GenBank accession numbers are provided in parentheses. Note that sterol-related ACAT plant proteins were omitted due insufficient annotation