Table 1.
Strains and plasmids used in this work
| Strains and plasmids | Relevant characteristics | Reference |
|---|---|---|
| Strains | ||
| BL21 Star (DE3) (reference) | F– ompT hsdS B(r–B, m–B) gal dcm rne131 (DE3) | Invitrogen |
| Δpgi | BL21 Star (DE3) Δpgi | (Siedler et al. 2011) |
| ΔpfkA | BL21 Star (DE3) ΔpfkA | (Siedler et al. 2011) |
| ΔpfkAΔpfkB | BL21 Star (DE3) Δpgi | (Siedler et al. 2011) |
| SS01 | BL21 Star (DE3) with pBtac-Lbadh | (Siedler et al. 2011) |
| SS02 | BL21 Star (DE3) Δpgi with pBtac-Lbadh | (Siedler et al. 2011) |
| SS03 | BL21 Star (DE3) ΔpfkA with pBtac-Lbadh | (Siedler et al. 2011) |
| SS04 | BL21 Star (DE3) ΔpfkAΔpfkB with pBtac-Lbadh | (Siedler et al. 2011) |
| Plasmids | ||
| pBTac1 | AmpR, Ptac, lacI; expression vector for E. coli | Boehringer Mannheim GmbH |
| pBTac-Lbadh | pBtac1 derivative with adh gene from Lactobacillus brevis | X-Zyme, (Ernst et al. 2005) |
| pVWEx1 | KanR; C. glutamicum/E. coli shuttle vector (P tac, lacI q; pHM1519, oriV C. glutamicum, oriV E. coli) | (Peters-Wendisch et al. 2001) |
| pVWEx1-glf-glk | pVWEx1 derivative with glf and glk genes from Zymomonas mobils | Eggeling et al. unpublished |