Table 2.
Growth rates, glucose uptake rates, and cell yields (gram cdw per gram glucose) of the reference strain E. coli BL21(DE3) and three deletion mutants used in this study
Strain | Growth rate μ (h−1) | Glucose uptake rate (mmol h−1 g−1cdw) | Cell yield YX/S (g g−1) |
---|---|---|---|
Reference | 0.60 ± 0.01 | 11.33 ± 0.02 | 0.36 ± 0.01 |
Δpgi | 0.11 ± 0.01 | 2.76 ± 0.09 | 0.28 ± 0.01 |
ΔpfkA | 0.18 ± 0.01 | 4.00 ± 0.08 | 0.28 ± 0.01 |
ΔpfkAΔpfkB | 0.01 ± 0.01 | nd | nd |
Mean values and standard deviations from at least three independent experiments are shown
nd not determined