Table 4.
Parameters for the whole-cell biotransformation of MAA to MHB with glucose as donor of reducing equivalents using the indicated E. coli strains
| Strain/Plasmid | MHB production rate | Glucose uptake rate | Yield |
|---|---|---|---|
| (mmol h−1−g−1cdw) | (mmol h−1 g−1cdw) | (molMHB mol−1Glucose) | |
| SS01/pVWEx1 | 8.5 ± 0.1 | 3.0 ± 0.1 | 2.9 ± 0.2 |
| SS01/pVWEx1-glf-glk | 8.6 ± 0.3 | 4.4 ± 0.2 | 1.9 ± 0.1 |
| SS02/pVWEx1 | 8.6 ± 0.3 | 2.3 ± 0.1 | 3.7 ± 0.2 |
| SS02/pVWEx1-glf-glk | 9.9 ± 0.4 | 3.0 ± 0.3 | 3.3 ± 0.3 |
| SS03/pVWEx1 | 8.6 ± 0.2 | 1.7 ± 0.2 | 4.7 ± 0.3 |
| SS03/pVWEx1-glf-glk | 10.3 ± 0.1 | 2.4 ± 0.3 | 4.4 ± 0.2 |
| SS04/pVWEx1 | 5.6 ± 0.3 | 1.0 ± 0.2 | 5.5 ± 0.3 |
| SS04/pVWEx1-glf-glk | 5.4 ± 0.4 | 1.4 ± 0.1 | 3.8 ± 0.4 |
The detailed conditions are described in “Materials and methods.” Mean values and standard deviations from at least three independent experiments are shown