Fig. 2.

Dual immunostained fibrillin-2 and fibrillin-1 in the outer annulus fibrosus of the bovine disc. (A) Parallel fibrillin-2 fibres (green) in an inplane lamella (IP) with the red arrow highlighting a long fibrillin-2 fibre (longer than 170 μm). (B–D) Typical dual immunostaining of a section, with (B) showing organisation of fibrillin-2 (green), (C) fibrillin-1 organisation (red), and (D) the merged image of (B) and (C). The figures indicate that fibrillin-2 is highly co-localised with fibrillin-1; together, they are densely organised at the interlamellae (white arrows) and at the boundaries of collagen bundles (yellow arrows). CS, cross-sectioned lamella; nCS, nearly cross-sectioned lamella; IP, inplane lamella; nIP, nearly inplane lamella.