Fig. 4.

Dual immunostained fibrillin-2 (A) and fibrillin-1 (B) in the inner annulus fibrosus. The figure shows typical dual staining of a section, with (A) showing fibrillin-2 (green) distribution, (B) fibrillin-1 (red) distribution and (C) a merged image of (A) and (B) where the cells are stained with DAPI (blue). The figures show more fibrillin-2 in the inter-territorial matrix (A: yellow arrows) where little fibrillin-1 is present (B). White arrow in (A) shows that fibrillin-2 is also concentrated around cells. (D) A higher magnification of a merged image showing a network of fibrillin-1 and -2 forming a chondron-like structure encircling the cell and connecting with the fibres at the inter-territorial matrix (white arrows).