Fig. 8.

Dual immunostained LTBP-2 and fibrillin-1 in the NP. A typical dual stained image at low magnification (A–C) and higher magnification (D–F) showing LTBP-2 (green: A and D) and fibrillin-1 (red: B and E). (C, F) Merged images of (A) and (B), and (D) and (E), respectively, with cell nuclei (blue) stained with DAPI. (A–C) LTBP-2 appears highly co-localised with fibrillin-1 and together they are distributed densely around the cells. (D–F) At a higher magnification, co-localised LTBP-2 and fibrillin-1 clearly encircle cells.