Table 1.
Fibrillin-1 | Fibrillin-2 | LTBP-2 | Elastin | |
---|---|---|---|---|
1st antibody | Mice anti-bovine fibrillin-1 from Abcam UK Ltd, UK, Cat. no: ab3090 | Rabbit anti-human fibrillin-2 antibody (cross-reacted with bovine) from Elastin Products Company, USA, Cat. no: PR225 | Rabbit anti-bovine LTBP-2 from Elastin Products Company, USA, Cat. no: PR410 | Mice anti-bovine elastin from Abcam UK Ltd, UK, Cat. No: ab9519 |
Dilution: 1 : 50 Buffer: 50 mm Tris–HCl plus 10 mm calcium acetate | Dilution: 1 : 50 | Dilution: 1 : 50 | Dilution: 1 : 5 | |
2nd antibody | Cy3-conjugated donkey anti-mouse IgG | DyLight-488- conjugated donkey anti- rabbit IgG | DyLight-488- conjugated donkey anti- rabbit IgG | Cy3-conjugated donkey anti-mouse IgG |
(Red) Stratech Scientific Ltd, UK, Product code: 715-165-151 Dilution: 1 : 100 | (Green) Stratech Scientific Ltd, Product code: 712-485-153 Dilution: 1 : 100 | (Green) Stratech Scientific Ltd, Product code: 712-485-153 Dilution: 1 : 100 | (Red) Stratech Scientific Ltd, UK, Product code: 715-165-151 Dilution: 1 : 100 |
Dilution with PBS unless otherwise indicated.