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. 2011 Nov 20;220(2):164–172. doi: 10.1111/j.1469-7580.2011.01455.x

Table 1.

List of antibodies used*

Fibrillin-1 Fibrillin-2 LTBP-2 Elastin
1st antibody Mice anti-bovine fibrillin-1 from Abcam UK Ltd, UK, Cat. no: ab3090 Rabbit anti-human fibrillin-2 antibody (cross-reacted with bovine) from Elastin Products Company, USA, Cat. no: PR225 Rabbit anti-bovine LTBP-2 from Elastin Products Company, USA, Cat. no: PR410 Mice anti-bovine elastin from Abcam UK Ltd, UK, Cat. No: ab9519
Dilution: 1 : 50 Buffer: 50 mm Tris–HCl plus 10 mm calcium acetate Dilution: 1 : 50 Dilution: 1 : 50 Dilution: 1 : 5
2nd antibody Cy3-conjugated donkey anti-mouse IgG DyLight-488- conjugated donkey anti- rabbit IgG DyLight-488- conjugated donkey anti- rabbit IgG Cy3-conjugated donkey anti-mouse IgG
(Red) Stratech Scientific Ltd, UK, Product code: 715-165-151 Dilution: 1 : 100 (Green) Stratech Scientific Ltd, Product code: 712-485-153 Dilution: 1 : 100 (Green) Stratech Scientific Ltd, Product code: 712-485-153 Dilution: 1 : 100 (Red) Stratech Scientific Ltd, UK, Product code: 715-165-151 Dilution: 1 : 100
*

Dilution with PBS unless otherwise indicated.