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Influence of magnesium on the secretion of proteins involved in calcification. Protein concentrations of MGP and BMP2 were quantified in the supernatants of BVSMCs by ELISA (A and B). Cells were incubated for 14 days under calcifying conditions either in the presence of physiological or elevated extracellular magnesium concentrations. Data shown are means and SD of eight replicates (a = difference versus BGP: P < 0.05; b = difference versus control: P < 0.05).
