Table 1.

Estimated Mg uptake by BVSMCs during 48 h incubation^a

	Initial Mg [mM]	Mg after 48 h [mM]	Δ Mg [mM]	Δ Mg [nmol]
Control	1.1	0.77 ± 0.02	0.33 ± 0.02	0.66 ± 0.05
BGP	1.1	0.75 ± 0.06	0.35 ± 0.06	0.69 ± 0.12
+2 mM Mg	2	0.84 ± 0.12	1.16 ± 0.12	2.23 ± 0.24
+3 mM Mg	3	1.62 ± 0.14	1.38 ± 0.14	2.76 ± 0.29

^aCalcification was induced in BVSMCs in the presence of physiological or increasing magnesium concentrations for 14 days. Magnesium uptake was estimated by calculating the differences of magnesium present in the media before and after incubation for 48 h with fresh media containing the same additives as before.