Table 1. PCR primers used for RT-PCR of m1–m5 mRNA.
Primer | Sequence (5′-3′) | Product Size (bp) |
---|---|---|
ml-a-upper | CCCATCATCACTTTTGGCACA | |
ml-a-lower | CTGCAGGGCGGCCAGCTCCCG | 123 |
ml-b-upper | CCTCTGCTGCCGCTGTTG | |
ml-b-lower | GGTGGGTGCCTGTGCTTCA | 175 |
m2-upper* | CACGAAACCTCTGACCTACCC | |
m2-lower* | TCTGACCY†GAMGAACCCAACTA | 686 |
m3-upper* | GTCTGGCTTGGGTCATCTCTT | |
m3-lower* | GCTGCTGCTGTGGTCTTGGTC | 433 |
m4-upper* | TGGGTCTTGTCCTTTGTGCT | |
m4-lower* | TTCATTGCCTGTCTGCTTTGTT | 587 |
m5-upper* | CTGGTCTCCTTCATCCTCTGG | |
m5-lower* | CCTGGGTTCTCTTTCCTGTTG | 394 |
PCR, polymerase chain reaction; RT, reverse transcriptase; bp, base pairs.
Sequences adapted from Wei et al. (27).
Mixed base code of CT; M, mixed base code of AC.