Table 1.

Dilution scheme for preparation of the calibration curve

Solution to be diluted, μg/mL	Dilution, v + v	Final concn, μg/mL
100	—a	100
100	1 + 1	50
100	1 + 4	20
100	1 + 9	10
50	1 + 9	5
20	1 + 9	2
10	1+ 9	1
5	1 + 9	0.5
2	1 + 9	0.2
1	1 + 9	0.1
0.5	1 + 9	0.05

^a— = Undiluted.