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. 2011 Oct 3;10(12):M111.011627. doi: 10.1074/mcp.M111.011627

Fig. 5.

Fig. 5.

N-terminal extension of a gene using peptides mapping upstream to an annotated start site. A, Two peptides were mapped to the upstream region of gene Rv2241. Gene prediction algorithms FgeneSB and GeneMark predicted presence of longer gene extending N-terminal of Rv2241 by 29 amino acids. M. tuberculosis CDC1551 ortholog supports the N-terminal extension. B, Protein sequence of modified gene model for Rv2241. Sequence in gray box indicates the extended N terminus. C, A representative MS/MS spectrum for the peptide HDLAQNSNSASEPDR is shown.