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Mice were subjected to 1 hr of ischemia followed by 24 hrs of reperfusion. A. Animals were examined at 24 hours of reperfusion for apoptosis using a TUNEL assay. B. Animals were examined at 24 hrs for the astroglial inflammatory marker GFAP. C. Quantitative analysis of apoptosis in the ischemic brain. D. Quantitative analysis of GFAP staining in the ischemic brain. *p<0.0001 for all groups compared to controls.
