Fig. 5.

Kinetics of CaM binding to the N-terminus of WT and S57D ACA8. Phases of the interaction between CaM and peptides 6His-¹M-I¹¹⁶ derived from WT and S57D ACA8 were registered by surface plasmon resonance spectroscopy, using different concentrations of bovine testes CaM (a=350 nM; b=200 nM; c=100 nM; d=50 nM) as the flowing analyte. The reported signal (RU) is the difference in resonance units between the signal recorded in the measuring cell, with immobilized peptide, and the signal recorded in the peptide-free reference cell.