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. 2011 Dec 5;63(3):1367–1380. doi: 10.1093/jxb/err366

Fig. 11.

Fig. 11.

ImmunoEM study of DV profiles in wortmannin-treated versus untreated developing mung bean cotyledons. Developing mung bean cotyledons were treated or untreated with wortmannin as indicated, followed by sample preparation for immunoEM labelling with anti-8S globulin antibodies and TEM observations. (A) Ultrathin sections were labelled with anti-8S globulin for immunogold study. Panels 1, 3, and 5 show examples of PM and Golgi/DVs in untreated control samples, whereas panels 2, 4, and 6 show examples of PM–DV fusion and DV aggregation/fusion in wortmannin-treated samples. Arrows indicate examples of possible DV–PM fusions (panels 2 and 4) or DV fusion/aggregation (panel 6) in wortmannin-treated samples. Scale bar=500 nm. (B) Analysis of average diameter size of DVs (in nm) in wortmannin-treated versus untreated developing mung bean cotyledons.