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. 2011 Dec 5;63(3):1367–1380. doi: 10.1093/jxb/err366

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — IEM analysis of distinct MVBs of 16 DAF developing mung bean seeds. Developing mung bean cotyledons at 16 DAF were collected, high-pressure frozen/freeze-substituted, and embedded in HM20. Ultrathin sections were then prepared, followed by IEM analysis using 8S globulin and VSR antibodies as indicated. Panel 1, an overview of labelling of anti-8S globulin on various organelles including the Golgi (GA), PSV, and the distinct MVB (also shown in panel 2). Panels 3 and 4, the 8S globulin-positive distinct MVBs in samples treated with wortmannin and BFA, respectively. Panels 5 and 6, distinct MVBs labelled with anti-VSR (panel 5) and double-labelled with anti-8S globulin and anti-VSR (panel 6). VSR, vacuolar sorting receptor; GA, Golgi apparatus; Mt, mitochondrion; PSV, protein storage vacuole; Wort, wortmannin; BFA, brefeldin A. Scale bars are included as indicated.