Table 2.
Organelle | Wortmannin | Average size (diameter, nm) | Standard deviation of organelle size | No. of organelles counted |
MVBs/PVCs in 8 DAF | Untreated | 350** | 71 | 30 |
Treated | 1101** | 294 | 30 | |
MVBs/PVCs in 16 DAF | Untreated | 349NS | 40 | 32 |
Treated | 344NS | 45 | 32 |
Significant differences between wortmannin-untreated and treated MVBs/PVCs were analysed using two-tailed paired t-test (**P < 0.001; NS, non-significant). Data were collected and analysed from three independent experiments.