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. 2011 Dec 7;63(3):1391–1404. doi: 10.1093/jxb/err383

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Expression analysis of seed maturation genes in essp4 mutant leaves. (A) RNA blot analysis of the expression of the five 2S genes in leaves of four sdg8 mutants grown for 14 d on MS agar. Wild-type (Col) leaves and siliques were used as negative and positive controls, respectively. The same amount of RNA was used for each blot. Elongation factor 1α was used as loading control. (B) Real-time quantitative RT-PCR (qRT-PCR) validation of the expression in sdg8-5 leaves of seed-related genes revealed in the DNA microarray analysis. RNAs from leaves of 14-day-old plants grown on MS agar were used for PCR. Only those validated by qRT-PCR are shown here. Wild-type (βCG_pro:GUS) RNA levels are designed as 1-fold. The expression of Actin-8 was used as an internal control. The mean and standard error (SE) were determined from three biological replicates. Bars represent SEs. (C) qRT-PCR analysis of ABI3, FUS3, LEC1, and LEC2 genes in seedlings (aerial portion) of sdg8-2 mutants grown for 14 d on MS agar. Wild-type (Col) RNA levels are designed as 1-fold. The expression of Actin-8 was used as an internal control. The mean and SE were determined from three biological replicates, each of which was conducted in triplicate.