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. 2011 Dec 3;63(3):1479–1493. doi: 10.1093/jxb/err392

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — Hydrogen peroxide in olive pistils excised from freely pollinated flowers detected by confocal laser scanning microscope. (A, B, C) Stereomicroscope images of olive flowers before pollination (St I), during pollination (St II), and after fertilization (St III). (D, E, F, G) Detection of H₂O₂ by DCF-DA in stigmas at different stages. Green fluorescence corresponding to H₂O₂ is detected in papillar cells mainly at stage I. (G) Pistil at stage I treated with ascorbate (ASC), a H₂O₂ scavenger. (H) Histogram showing fluorescence quantified in total images of the stigmal lobules in arbitrary units using LAS AF Leica software. Different letters indicate significant difference at P <0.05 as determined by Duncan's multiple-range test. PC, papillar cells. (A, B, C) Bars=2 mm; (D, E, F, G) bars=50 μm. (This figure is available in colour at JXB online.)