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. 2011 Dec 3;63(3):1479–1493. doi: 10.1093/jxb/err392

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 6. — NO involvement in stigma development. (A, B, C) Flower buds were emasculated during stage I, sprayed with a NO donor (SNP), and bagged to avoid the arrival of pollen grains onto their surface, after which samples were collected at stage III. (D, E, F) Flower buds were emasculated and bagged during stage I without adding SNP, after which samples were collected at stage III. (A, D) H₂O₂ was detected by DCF-DA. (B, E) O₂^˙− was detected by DHE. (C, F) Cell death was detected by trypan blue staining. Pc, papillar cells. Bars=50 μm. (This figure is available in colour at JXB online.)