Table 1.
Requirement for preparation of mammary gland.
| MEC 1 | SC2 | ECM | N 3 | x/T 4 | Outcome |
|---|---|---|---|---|---|
| Humanized mammary fat pads | |||||
| MCF7 (2 × 105) | hTert MSC (2 × 105) | Col I plus matrigel (3 : 1) | 8 | 0/16 | Solid tumor |
| MCF7/CEACAM1-4S (2 × 105) | hTert MSC (2 × 105) | Col I plus matrigel (3 : 1) | 8 | 14/16* | Differentiation into striated epithelium |
|
| |||||
| Normal mouse mammary fat pads | |||||
| MCF7 (2 × 105) | hTert MSC (2X105) | Col I plus matrigel (3 : 1) | 8 | 0/16 | Solid tumor |
| MCF7/CEACAM1-4S (2 × 105) | hTert MSC (2X105) | Col I plus matrigel (3 : 1) | 8 | 14/15* | Differentiation into striated epithelium |
1Mammary epithelial cell line (number of cells injected), 2Stromal cell line (number of cells injected), 3Number of animals, 4Number of xenografts exhibiting striated epithelial structure (x)/Total number of xenografts (T), *indicates statistical significance (P < .01) for differentiation of xenografts, when compared with control group (MCF7).