Figure 1 .

Two methods for genome-wide analysis of entry into and recovery from senescence. An est1::natMX strain (DLY5026) was crossed to the deletion mutant collection and double mutants were selected using SGA procedures. (A) Double mutants were passaged in 96-well plate liquid cultures, and fitness was monitored after spotting onto solid agar plates at 384 spot cell density for a total of 16 passages. (B) Cultures were serially pinned at 1536 colonies per plate and photographed for 22 passages.