Figure 4 .

Three senescence profile archetypes. Mean density profiles (MDP) were estimated for each of 4300 gene deletions combined with est1∆. Pearson’s correlation coefficient (r) was calculated for each mutation compared with his3∆, rad52∆, and rif1∆ mutations, representing three distinct senescence curve archetypes. (A) A heat map showing the MDP of the mutations having the 14 highest correlations with that of his3∆ est1∆. (B) All 3035 MDPs (pink) correlating strongly (r > 0.85) are shown along with the MDP of the archetype his3∆ est1∆ (red), and the mean curve of the 3036 strongly correlating MDPs (black). (C) A heat map showing the MDP of the mutations having the 20 highest correlations with that of rad52∆ est1∆. To construct this group, we only included strains whose culture size at passage 2 was less than C^s = 1680, where C^s = C^ave – 0.5*C^SD, C^ave is the average of all double mutant culture sizes at passage 2, C^SD is the culture size standard deviation at that passage. (D) All 208 MDPs (pink) correlating strongly (r > 0.5, culture size < C^s) with the MDP of rad52∆ est1∆ (red), together with the average of the 209 strongly correlating MDPs (black). (E) A heat map showing the MDP of the mutations having the 14 highest correlations with that of rif1∆ est1∆. (F) Twenty gene disruptions whose MDPs (pink) correlate best with the MDP of rif1∆ est1∆ (red), together with the average of the 21 most strongly correlating MDPs (black). (G) Scale for heat maps.