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P[L181PRE] construct and eye colors. (A) P[L181PRE] is identical to P[181PRE] except the 181-bp en PRE is flanked by loxP sites (L). PSS is the number of lines with pairing-sensitive silencing/the total number of viable lines obtained. (B) Eyes from 2-day-old males of lines P[L181PRE]C2A (L181) and P[L]C2A (L) are shown. Note that the P[L]/+ eyes are darker than the P[L181PRE]/+ eyes.
