Figure 2 .

pIB485-like enterotoxin plasmids with or without ΔTn552. (A) Diagram of pIB485-like plasmid SAP060A (open reading frames in gray), positions 2210–8928, showing the insert position of the 4278 bp ΔTn552 (hatched) in the sin res site (Rowland et al. 2002) in the other pIB485-like plasmids (Table 6). The ΔTn552 (hatched) insert has flanking 10 bp direct repeats (right-facing gray arrowheads) of res site DNA, likely resulting from transposition. An inverted repeat of that same 10 bp insertion site is also present upstream from sin, as part of the predicted res site (left-facing gray arrowhead). The black arrows show predicted open reading frames. (B) The sequence details of the ΔTn552 inserted in the sin res site. The sequence without the insertion is SAP060A positions 2454–2528. The other pIB485-like plasmid sequences contain ΔTn552, and the nucleotides shown are identical in all. The gray arrows show the 10 bp direct and inverted repeats.