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Microinjection plate and needle for transgenesis. (A) Microinjection plate cast using an ad hoc built plastic mold. Embryos selected for injection are aligned along the trenches and pierced with borosilicate needles. Up to 40 embryos could fit each trench. (B) Plastic mold design and measures. (C) Comparison between the microinjection needle for zebrafish (top) and N. furzeri (bottom). N. furzeri needles are sturdier than zebrafish needles, allowing better chorion piercing.
