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Expression of GFP in pCska-gfp Tol2 transgenic N. furzeri. GFP expression in live noninjected (top row), injected P0 fish (second row), and the F1 (third row), and F2 (bottom row) progeny of GFP-positive N. furzeri. Pictures were taken 12 days postfertilization for embryos, and 5 days posthatching for fry. Bright field and GFP images are shown for embryos. Scale bar: 1 mm. GFP, green fluorescent protein.
