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. 2012 Jan 1;2(1):59–69. doi: 10.1534/g3.111.001586

Figure 4 .

Figure 4 

Western analysis and transformation rescue confirm that e(cac)2902 is dp32. (A) Western analysis of fly head homogenates prepared from WT and dp32EC1, as well as the dp32EC1 mutant expressing wild-type dP32 in the nervous system (dp32EC1/Df rescue). A prominent band at ∼26 kD is recognized by an anti-dP32 antibody. This band is absent in the mutant and elevated after neural expression of UAS-dp32 in the dp32EC1 mutant. Tubulin (TUB) was used as an internal loading control. (B) Presynaptic expression of wild-type dP32 rescues the dp32EC1 paralytic phenotype. Behavioral data for WT and dp32EC1/Df are the same as those shown in Figure 1. Behavioral tests were truncated after 20 min. These results confirm that TS paralysis in dp32EC1 results from disruption of dP32.