Figure 6 .

Homomultimeric interactions of dP32: dP32-EGFP and endogenous dP32 coimmunoprecipitate from fly head lysate. An anti-GFP antibody was used to precipitate dP32-EGFP from head lysate of flies expressing dP32-EGFP in the nervous system or control flies (WT) lacking the transgene. Immunoprecipitation (IP) of dP32-EGFP (A) and co-IP of endogenous dP32 (B) was demonstrated by Western blotting using anti-GFP and anti-dP32 antibodies, respectively. Specific co-IP of dP32 was observed only in samples expressing dP32-EGFP, indicating homomultimeric association of dP32-EGFP and endogenous dP32 subunits.