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. 2012 Jan 1;2(1):83–97. doi: 10.1534/g3.111.001255

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Copyright © 2012 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Quantitative analysis of gene copy number of AD hybrid isolates. Heterozygous loci of AD hybrid isolates have two copies of a gene, one from the serotype A parent and the other one from the serotype D parent. The primers used for quantitative analysis of the gene copy number of the AD hybrid isolates are located in regions conserved between serotype A and D and can amplify both serotype A and D loci. The primers target three loci on Chr 1, Chr 4, and Chr 9. On the basis of CGH, strain IUM92-6198 has lost serotype D Chr 9, and strains KW5 and CDC228 have lost serotype D Chr 1; however, these strains have an identical copy number of genes on Chr 1, Chr 4, and Chr 9. This finding indicates chromosome duplication of the remaining chromosomes after chromosome loss from one parental serotype in the AD hybrid.