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. 2012 Jan 1;2(1):79–82. doi: 10.1534/g3.111.001321

Figure 2 .

Figure 2 

Vectors accurately report expression pattern from a Notch responsive element. (A) Basal expression from pGreenRabbit (GR) integrated at platform 51D. (B–E) Expression from indicated reporter vectors driven by the Notch responsive element (NRE) in the wing pouch of third instar larvae. NRE-lacZ (B, magenta; B′′, white) is a previously reported Notch reporter (Furriols and Bray 2001). The same NRE was used to generate NRE-GreenRabbit (NRE-GRins; B, green; B′, white); NRE-RedRabbit (NRE-RRins; C); NRE-BlueRabbit (NRE-BRins; D); and NRE-VenusRabbit (NRE-VRins; E). NRE-VRins was imaged with 10× excitation. (F) Notch-RNAi expression in the posterior compartment (green braces; driven using en-Gal4) eliminates expression from NRE-GRins. (G, H) Basal expression from pGR integrated at platform 51D in the larval brain (G) and trachea (H). (I, J) Expression from NRE-GRins in the larval brain (I) and trachea (J). Suffix “ins” indicates that the constructs contained insulators. (K) Comparison of expression levels from independent transgenic lines (average pixel intensity in the wing pouch measured using ImageJ). NRE-RR insertions at the same platform (86Fb) produce similar expression levels. NRE-lacZ insertions generated using p-element transgenesis are expressed at varying levels. At least five discs were quantified per genotype. Error bars show standard error of the mean. Primary antibodies were rabbit α-GFP (Molecular Probes, 1/500) (A, B, and E–J); rabbit α-dsRed (ClonTech, 1/50) (C); and mouse α-βGalactosidase (Developmental Studies Hybridoma Bank, 1/20) (B and D).