Table I.

PCR primer sequence for preparation of ST6gal-I Cys-residue mutants.

Mutant	Mutation	Primer for the first PCR
name		Forward	Reverse	Plasmid names
139C/S	195C→S	5′-GCGCTGCGCTCCCACCTTCGAGAC	5′-GTCTCGAAGGTGGCAGCGCAGCGC	pcDSA-ST6Gal-I-195C/S
139C/A	195C→A	5′-GCGCTGCGCGCCCACCTTCGAGAC	5′-GTCTCGAAGGTGGGCGCGCAGCGC	pcDSA-ST6Gal-I-195C/A
181C/S	237C→S	5′-CCTTGGCATAAGTCTGCCGTCGTG	5′-CACGACGGCAGACTTATGCCAAGG	pcDSA-ST6Gal-I-237C/S
350C/S	406C→S	5′-CAGATGTGTCCTACTATCACCAG	5′-CTGGTGATAGTAGGACACATCTG	pcDSA-ST6Gal-I-406C/S
350C/A	406C→A	5′-CAGATGTGGCCTACTATCACCAG	5′-CTGGTGATAGTAGGCCACATCTG	pcDSA-ST6Gal-I-406C/A

Underline shows the sequence corresponding to the mutation.