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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Microbiol. 2012 Jan 29;83(4):840–855. doi: 10.1111/j.1365-2958.2012.07968.x

Fig. 2. Purification and solution structure of recombinant Bcam0581 protein.

Fig. 2

A. Size exclusion chromatography of the His-tagged Bcam0581 protein expressed in E. coli. The inset is a 10% SDS-PAGE analysis of the proteins of the peaks 1 and 2 where M, is a protein standard marker (BioRad). The elution peaks of the molecular weight standards are given at the top of the panel. B. Chemical cross-linking analyses of the purified Bcam0581 protein where EGS denotes ethylene glycol bis succinimidylsuccinate (Experimental procedures). The samples were separated by 10% SDS-PAGE where M is the Precision Plus Protein Standard (BioRad).