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. 2011 Oct 14;21(2):384–393. doi: 10.1093/hmg/ddr473

Figure 2.

Figure 2.

Characterization of corneal endothelium and DM in Col8a2Q455K/Q455K (MUT) mice and human subjects. (A–F) Clinical confocal microscopy of 5-month WT endothelium (A) showing characteristic polygonal monolayer of cells with regular size and shape and 5-month MUT endothelium (B) showing increased variability in cell size and shape with DM excrescences (guttae,*) which are highly characteristic of FECD. Ten-month WT endothelium (C) showing typical cellular appearance and 10-month MUT endothelium (D) showing increased irregularity of cell size and shape with increased accumulation of guttae (*). Endothelium from a normal 54-year-old Caucasian female (E) and from a human FECD patient heterozygous for the COL8A2 Q455K mutation [F, reprinted with permission (5)] showing endothelial cell irregularities and guttae (*). Scale bar = 60 μm. (G) Central CEC density and (H) % hexagonal cells for 5-month (n= 8) and 10-month (n= 11) WT and 5-month (n= 10) and 10-month (n= 8) MUT mice. Box and whisker plots represent mean, 25th and 75th percentile values, and minimum and maximum values. (G) *P< 0.0001 compared with WT group of same age. ^P= 0.0191 compared with 5-month MUT group. (H) *P = 0.0009 for 5-month MUT and P < 0.0001 for 10-month MUT compared with WT group of same age.