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. 2011 Oct 14;21(2):384–393. doi: 10.1093/hmg/ddr473

Figure 3.

Figure 3.

Periodic acid-Schiff stain of formalin-fixed, paraffin-embedded sections of 10-month MUT (A and B) and WT (C and D) corneas (scale bars (A and C) = 30 μm, (B and D) = 10 μm]. (A and C) Stratified corneal epithelium (epi) at the bottom of panels and lamellar corneal stroma (str) appear comparable between MUT and WT genotypes. MUT DM shows two guttae adjacent to an endothelial cell nucleus (A, triangles). DM in MUT versus WT mice appears similar in thickness (B and D, arrows). (E–H) TEM of DM and endothelial cell from 5-month MUT (E and G) and 10-month MUT (F and H) mice [scale bars (E and F) = 2 μm, (G and H) = 500 nm]. DM gutta from a 5-month MUT (E) shows sharper, discontinuous edges with internal ultrastructural features compared with the more rounded, amorphous gutta from a 10-month MUT (F). In both (E) and (F), guttae appear to be similar in size to adjacent endothelial cells (insets). The predominant cellular ultrastructural abnormality in 5-month MUT endothelium (G) is elongated and dilated RER. In 10-month MUT endothelium (H), the RER is more severely dilated, and abnormal mitochondria (*) are present.