Skip to main content
. 2011 Oct 14;21(2):384–393. doi: 10.1093/hmg/ddr473

Figure 5.

Figure 5.

Assessment of UPR-associated apoptosis in corneal endothelium of Col8a2Q455K/Q455K (MUT) mice. (A) Immunofluorescence of UPR-associated apoptosis markers shows increased signal in the endothelium (arrows) of 10-month (caspase 12) and 20-month (cleaved caspase 9) MUT versus WT mice. (B) Quantitative RT-PCR shows increased caspase 12 in endothelium of 12-month MUT versus WT mice (*P= 0.0003). (C) Cleaved caspase 9 immunofluorescence shows increased mean signal intensity in endothelium of 20-month MUT versus WT mice (*P= 0.0063). (D) TUNEL staining of 20-month MUT endothelium shows apoptotic cells (arrows) individually and in clusters compared with no apoptosis in WT endothelium. *TUNEL-negative nucleus of an adjacent corneal stromal keratocyte.