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NMNAT reduces the total vacuole volume per brain induced by hTau overexpression. Overexpression of both wild-type and enzyme-deficient NMNAT reduces total vacuole volume per brain. Vacuole volume was quantified by using the FluoView10-ASW (Olympus) software, assuming each vacuole to be spherical in form. Numbers at the bottom of the bars indicate the number of brains quantified per age per genotype. *P< 0.001, **P< 0.05.
